Features of mono‐ and multinucleated bone resorbing cells of the zebrafish Danio rerio and their contribution to skeletal development, remodeling, and growth

  title={Features of mono‐ and multinucleated bone resorbing cells of the zebrafish Danio rerio and their contribution to skeletal development, remodeling, and growth},
  author={Paul Eckhard Witten and Arne Hansen and Brian K. Hall},
  journal={Journal of Morphology},
To provide basic data about bone resorbing cells in the skeleton during the life cycle of Danio rerio, larvae, juveniles, and adults (divided into six age groups) were studied by histological procedures and by demonstration of the osteoclast marker enzyme tartrate‐resistant acid phosphatase (TRAP). Special attention was paid to the lower jaw, which is a standard element for fish bone studies. The presence of osteoclasts at endosteal surfaces of growing bones of all animals older than 20 days… 
Functional bone histology of zebrafish reveals two types of endochondral ossification, different types of osteoblast clusters and a new bone type
Insight is provided on how osteogenesis takes place in zebrafish, and especially on the transition from cellular to acellular bones, which leads to a deeper understanding of the functional histological composition of adult zebra fish bones.
Osteoclast-like Cells in Early Zebrafish Embryos
Findings show that cells expressing a range of osteoclast markers are present in early larvae and can be activated by the addition of osteOClastogenic compounds.
An adult osteopetrosis model in medaka reveals the importance of osteoclast function for bone remodeling in teleost fish.
It is of crucial importance from the point of view of understanding vertebrate hematopoiesis to investigate which genes when intercepted produce osteopetrosis because these would be the genes that are controlling the process of formation of bone marrow as an organ.
Restriction of retinoic acid activity by Cyp26b1 is required for proper timing and patterning of osteogenesis during zebrafish development
Results indicate that temporal and spatial restriction of RA signaling by Cyp26 enzymes is required to attenuate osteoblast maturation and/or activity in vivo, and cyp26b1 mutants may serve as a model to study the etiology of human vertebral disorders such as Klippel-Feil anomaly.
Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth
It is demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted, and tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment.
The zebrafish as a model for tissue regeneration and bone remodelling
The role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adultZebrafish scales is studied to understand the effect of early exposure to stress on wound healing and tissue Remodelling.


Growth requires bone resorption at particular skeletal elements in a teleost fish with acellular bone (Oreochromis niloticus, Teleostei: Cichlidae)
It is reported here about bone resorption with regard to growth in the tilapia Oreochromis niloticus, and problems of osteoclast identification in bony fish are discussed.
Bone resorption and bone remodelling in juvenile carp, Cyprinus carpio L.
If and how bone resorption contributes to the skeletal development of carp, Cyprinus carpio L., a teleost species with ‘normal’ osteocyte-containing (cellular) bone is clarified, and the occurrence of bone remodelling suggest that the endoskeleton of juvenile carp might be involved in mineral metabolism.
Enzyme histochemical characteristics of osteoblasts and mononucleated osteoclasts in a teleost fish with acellular bone (Oreochromis niloticus, Cichlidae)
Abstract.Bone resorption by mononucleated cells was studied in the acellular bone of a teleost fish (Oreochromis niloticus) by histological and enzyme histochemical observations and by transmission
Stage‐specific expression patterns of alkaline phosphatase during development of the first arch skeleton in inbred C57BL/6 mouse embryos
3‐D reconstruction of expression in Meckel's cartilage revealed that the chondrocytes of Meckels cartilage which express alkaline phosphatase and the matrix of which undergoes mineralisation are those surrounded by the alkalineosphatase‐positive dentary ramus, suggesting that progenitor cells of the processes, dentaries and secondary cartilages all originate from a common pool.
Cellular biology of bone-resorbing cells.
  • N. Athanasou
  • Biology
    The Journal of bone and joint surgery. American volume
  • 1996
The origin and formation of osteoclasts will be considered, along with mechanisms governing their recruitment, activation, and function, and the manner in which these mechanisms contribute to osteoclastic bone resorption under various pathological conditions as well as the possible roles of other cells (both skeletal and extraskeletal in origin).
Ultrastructure of the osteogenesis of acellular vertebral bone in the Japanese medaka, Oryzias latipes (Teleostei, Cyprinidontidae).
An ultrastructural study by transmission electron microscopy (TEM) of the vertebrae of embryonic, larval, juvenile and mature medaka shows that each vertebra consists of a core of notochordal cells
Development of the cranium and paired fins in the zebrafish Danio rerio (Ostariophysi, Cyprinidae)
Developmental information on the structure and composition of the zebrafish skull, pectoral, and pelvic girdle, and paired fins from a large series of cleared and Alizarin red‐stained specimens is provided.
Scale development in zebrafish (Danio rerio)
Because of their late formation during ontogeny and of their regular pattern of development, scales in zebrafish represent a good model for further investigations on the general mechanisms of epithelial‐mesenchymal interactions during dermal skeleton development, and in particular for the study of the gene expression patterns.
Immunohistochemical identification of a vacuolar proton pump (V‐ATPase) in bone‐resorbing cells of an advanced teleost species, Oreochromis niloticus
Co-expression of V- ATPase, TRAP and TraATPase in fish osteoclasts is demonstrated for the first time and indicates that they function as active bone-resorbing cells.