The study explored the perception and acoustic features of prosodic hierarchy boundaries in Uygur and Mandarin Chinese. The results of the perception study showed that native speakers of Mandarin Chinese were more sensitive to prosodic hierarchy boundaries than native speakers of Uygur. The results of a detailed acoustic analysis indicated that the acoustic features of Uygur prosodic hierarchy boundaries were rather complicated than those of Mandarin Chinese. The pauses, the lengthening of pre-boundary syllable and the pitch variation were the acoustic correlates of prosodic hierarchy boundaries in Uygur. However, the pauses, the minimum pitch of preboundary and post-boundary syllables differed across the prosodic hierarchy boundaries in Mandarin Chinese. In addition, disyllabic words and trisyllabic words occur most frequently as prosodic words in Mandarin Chinese and Uygur respectively. The results of the present study indicated the influence of language typology on speech perception and acoustic features of prosodic hierarchy boundaries.