Feature extraction for DNA base-calling using NNLS

@article{AndradeCetto2005FeatureEF,
  title={Feature extraction for DNA base-calling using NNLS},
  author={L. Andrade-Cetto and E. Manolakos},
  journal={IEEE/SP 13th Workshop on Statistical Signal Processing, 2005},
  year={2005},
  pages={1408-1413}
}
We present new algorithms that can be used to extract features from a DNA chromatogram prior to base-calling. The algorithms assume that the inter-base distance has already been equalized using methods such as those presented in L. Andrade-Cetto and E. Manolakos (2005). We show first how a good estimate of the peak diffusion (spread) can be calculated from the raw trace and without having to known the underlying base sequence. Using the estimated inter-peak distance and peak spread parameters a… CONTINUE READING