Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing γ-linolenic acid

  title={Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing $\gamma$-linolenic acid},
  author={Douglas R. Tocher and John Gordon Bell and James R. Dick and John R. Sargent},
The effects of different dietary oils on the fatty acid compositions of liver phospholipids and the desaturation and elongation of [1-14C]18∶3n−3 and [1-14C]18∶2n−6 were investigated in isolated hepatocytes from Atlantic salmon. Atlantic salmon smolts were fed diets containing either a standard fish oil (FO) as a control diet, a 1∶1 blend of Southern Hemisphere marine oil and tuna orbital oil (MO/TO), sunflower oil (SO), borage oil (BO), or oliver oil (OO) for 12 wk. The SO and BO diets… 
Polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation and the effects of dietary linseed and rapeseed oils
The present study showed that during parr-smolt transformation in Atlantic salmon there is a pre-adaptive increase in hepatocyte fatty acyl desaturation/elongation activities that is controlled primarily by environmental factors such as photoperiod and temperature but that can also be significantly modulated by diet.
Effects of diets containing linseed oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes in Atlantic salmon (Salmo salar)
Replacing fish oil with 18:3n-3-rich linseed oil may enable salmon to maintain the levels of tissue n-3HUFA levels through a combination of increased desaturation activity and increased substrate fatty acid provision.
Influence of partial substitution of dietary fish oil by vegetable oils on the metabolism of [1-14C] 18:3n-3 in isolated hepatocytes of European sea bass (Dicentrarchus labrax L.)
The present study demonstrated that the European sea bass, as a carnivorous marine fish, presented a ‘marine’ fish pattern in the metabolism of 18:3n-3 to 20:5 n-3 and 22:6n- 3 and nutritional regulation of hepatocyte fatty acid desaturation was minimal, and dietary vegetable oils did not increase desaturase activities.
Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.).
Effects of dietary lipid level and vegetable oil on fatty acid metabolism in Atlantic salmon (Salmo salar L.) over the whole production cycle
The changes in fatty acid metabolism induced by high dietary oil and VO replacement contribute to altered flesh lipid content and fatty acid compositions, and so merit continued investigation to minimize any negative impacts that sustainable, environmentally-friendly and cost-effective aquaculture diets could have in the future.
Effect of dietary echium oil on growth, fatty acid composition and metabolism, gill prostaglandin production and macrophage activity in Atlantic cod (Gadus morhua L.)
Testing the hypotheses that dietary EO would increase tissue 20:4n-3 and 20:3n-6 and modulate immune function and eicosanoid production in juvenile Atlantic cod fed a diet where fish oil (FO) was replaced by EO found no significant differences in growth and feed efficiency, hepato-somatic index, percentages of liver and flesh lipids and lipid class compositions for cod fed FO and EO.


The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation
New information is provided on the changes in lipid metabolism which accompany parr-smolt transformation and it is suggested that diets which have a fatty acid composition more similar to that in aquatic invertebrates may be beneficial in effecting successful seawater adaptation.
Effects of dietary borage oil [enriched in γ-linolenic acid,18:3(n-6)] or marine fish oil [enriched in eicosapentaenoic acid,20:5(n-3)] on growth, mortalities, liver histopathology and lipid composition of juvenile turbot (Scophthalmus maximus)
The carcass composition of turbot can be altered, by means of dietary lipids, to contain increased levels of EPA and DHGLA which would be of potential benefit in human as well as in fish nutrition, however, caution should be exercised when using very highly unsaturated oils relatively rich in EPA which may generate histopathological lesions in the fish.
Effect of dietary cholesterol and/or omega 3 fatty acids on lipid composition and delta 5-desaturase activity of rat liver microsomes.
Feeding fish oil appears to prevent both the inhibition of 20:4 omega 6 biosynthesis and the accumulation of cholesterol ester that were apparent when 2% cholesterol was added to either beef tallow or linseed oil diets.
The conversion of linoleic acid and linolenic acid to longer chain polyunsaturated fatty acids by Tilapia (Oreochromis) nilotica in vivo
It is concluded that T. nilotica is capable of elongating and desaturating both 18:2 (n-6) and 18:3 ( n-3), but that this conversion is suppressed by dietary longer chain PUFA.
Lipids of arctic charr,Salvelinus alpinus (L.) II. Influence of dietary fatty acids on the elongation and desaturation of linoleic and linolenic acid
  • R. OlsenE. Ringø
  • Environmental Science, Biology
    Fish Physiology and Biochemistry
  • 2005
The incorporation of radiolabelled fatty acids into polar lipids of fish fed a commercial diet was very low, and the desaturation neglectible in both polar and neutral lipids, showing that Arctic charr under culture conditions do not convert short chain PUFA to longer chain metabolites.
Delta 6-desaturase activity in liver microsomes of rats fed diets enriched with cholesterol and/or omega 3 fatty acids.
It is suggested that the decrease in prostaglandin E2, thromboxane and prostacyclin levels generally observed after fish-oil consumption may be at least partly due to inhibition of C20:4, omega 6-fatty-acid synthesis from C18:2, Omega 6 fatty acid; and consumption of fish oil prevents the further decrease by dietary cholesterol that is apparent when cholesterol is fed in combination with diets high in saturated fat.