Fatty acid binding to bovine serum albumin prevents formation of intermediate during denaturation.

@article{Ahmad1995FattyAB,
  title={Fatty acid binding to bovine serum albumin prevents formation of intermediate during denaturation.},
  author={Naveed Ahmad and Mohammad A Qasim},
  journal={European journal of biochemistry},
  year={1995},
  volume={227 1-2},
  pages={563-5}
}
Urea-induced denaturation of defatted BSA and BSA containing six mol palmitic acid/mol protein (fatted BSA) has been studied by the techniques of UV difference spectroscopy and fluorescence spectroscopy. The notable differences between the two transitions are a shift in the transition of fatted BSA to a higher urea concentration and a three-state denaturation transition in defatted BSA compared to an apparent two-state denaturation transition in fatted BSA. The stable denaturation intermediate… CONTINUE READING