Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells.

@article{Kumagai2001FateON,
  title={Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells.},
  author={Fumi Kumagai and Arata Yoneda and Taichiro Tomida and Toshio Sano and Toshiyuki Nagata and Seiichiro Hasezawa},
  journal={Plant & cell physiology},
  year={2001},
  volume={42 7},
  pages={723-32}
}
Transgenic BY-2 cells stably expressing a GFP (green fluorescent protein)-tubulin fusion protein (BY-GT16) were subcultured in a modified Linsmaier and Skoog medium. The BY-GT16 cells could be synchronized by aphidicolin and the dynamics of their microtubules (MTs) were monitored by the confocal laser scanning microscopy (CLSM). We have succeeded in… CONTINUE READING