Fate of HIV-1 cDNA intermediates during reverse transcription is dictated by transcription initiation site of virus genomic RNA

@inproceedings{Masuda2015FateOH,
  title={Fate of HIV-1 cDNA intermediates during reverse transcription is dictated by transcription initiation site of virus genomic RNA},
  author={Takao Masuda and Yoko Sato and Yu-Lun Huang and Satoshi Koi and Tatsuro Takahata and Atsuhiko Hasegawa and Gota Kawai and Mari Kannagi},
  booktitle={Scientific reports},
  year={2015}
}
Retroviral reverse transcription is accomplished by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Here, we revealed an unprecedented role of 5'-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based on current consensus for HIV-1 transcription initiation site, HIV-1 transcripts possess a single G at 5'-ends (G1-form). However, we found that HIV-1 transcripts with additional Gs at 5'-ends (G2- and G3-forms) were abundantly expressed in… CONTINUE READING

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Showing 1-10 of 53 references

Differential contribution of basic residues to HIV-1 nucleocapsid protein's nucleic acid chaperone function and retroviral replication

  • H Wu
  • Nucleic Acids Res
  • 2014

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