Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes.

@article{Tchkonia2002FatDO,
  title={Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes.},
  author={Tamara Tchkonia and N. G. Giorgadze and Tamar Pirtskhalava and Yourka D. Tchoukalova and Iordanes Karagiannides and Robert Armour Forse and Matthew DePonte and Michael Stevenson and Wen Guo and Jianrong Han and Gerri Waloga and Timothy L. Lash and Michael D. Jensen and James L. Kirkland},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  year={2002},
  volume={282 5},
  pages={
          R1286-96
        }
}
Fat distribution varies among individuals with similar body fat content. Innate differences in adipose cell characteristics may contribute because lipid accumulation and lipogenic enzyme activities vary among preadipocytes cultured from different fat depots. We determined expression of the adipogenic transcription factors peroxisome proliferator activated receptor-gamma (PPAR-gamma) and CCAAT/enhancer binding protein-alpha (C/EBP-alpha) and their targets in abdominal subcutaneous, mesenteric… 
View on PubMed
ajpregu.physiology.org
246 Citations
Highly Influential Citations
8
Background Citations
92
Methods Citations
18
Results Citations
18

246 Citations

Depot specific differences during adipogenesis of porcine stromal‐vascular cells

Fat Depot–Specific Characteristics Are Retained in Strains Derived From Single Human Preadipocytes

Despite dividing 40 population doublings, hTERT strains derived from single preadipocytes retained fat depot–specific cell dynamic characteristics, consistent with heritable processes contributing to regional variation in fat tissue function.

Low abdominal subcutaneous preadipocyte adipogenesis is associated with visceral obesity, visceral adipocyte hypertrophy, and a dysmetabolic state

Low abdominal subcutaneous preadipocyte differentiation capacity in vitro is associated with visceral obesity, visceral adipocyte hypertrophy, and a dysmetabolic state.

Preferential expression of PAPPA in human preadipocytes from omental fat.

Investigation of the factors regulating PAPPA expression focusing on proinflammatory cytokines and resveratrol that have been shown to have negative and positive effects, respectively, on metabolism and diet-induced obesity found that Depot-specific P APPA expression in human preadipocytes may contribute to a depot-specific function.

Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns.

It is found that genome-wide expression profiles of primary preadipocytes cultured in parallel from abdominal subcutaneous, mesenteric, and omental fat depots were distinct, consistent with differentFat depots being separate mini-organs.

Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots.

Fat depots vary in function and size. The preadipocytes that fat cells develop from exhibit distinct regional characteristics that persist in culture. Human abdominal subcutaneous cultured…

Depot‐Specific Differences in Adipogenic Progenitor Abundance and Proliferative Response to High‐Fat Diet

It is shown that in mice fed a high‐fat diet, visceral fat grows mostly by hypertrophy and subcutaneous fat (SAT) by hyperplasia, providing a rationale for the different effects of specific adipose depots on metabolic health and the first evidence that dietary inputs can modulate the proliferation of adipogenic progenitors in adults.

Measuring committed preadipocytes in human adipose tissue from severely obese patients by using adipocyte fatty acid binding protein.

It is documented that adipocyte fatty acid binding protein (aP2) is expressed in human preadipocyte clones capable of replication, indicating that can be used as a marker of committed preadendipocytes in the stromovascular fraction of fresh human adipose tissue.

Regulation of adipogenesis by paracrine factors from adipose stromal-vascular fraction - a link to fat depot-specific differences.

Effects of dihydrotestosterone on differentiation and proliferation of human mesenchymal stem cells and preadipocytes

...

References

SHOWING 1-10 OF 61 REFERENCES

Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes.

Carnitine palmitoyltransferase 1 activity increased during differentiation and was higher in perirenal than in epididymal preadipocytes, particularly the muscle isoform.

Altered expression of C/EBP family members results in decreased adipogenesis with aging.

Processes intrinsic to adipose cells involving changes in C/EBP family members contribute to impaired adipogenesis and altered fat tissue function with aging, and are potentially reversible.

Effects of fat depot site on differentiation-dependent gene expression in rat preadipocytes.

  • J. KirklandC. HollenbergW. Gillon
  • Biology
    International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity
  • 1996
Levels of differentiation-dependent mRNAs studied in differentiating cultured rat epididymal and perirenal preadipocytes and in fat cells isolated from these depots indicated mechanisms which underlie site-dependent variability in adipose function may be intrinsic and could become evident midway through the differentiation process.

Human Preadipocytes Display a Depot-Specific Susceptibility to Apoptosis

Omental preadipocytes are more susceptible to two different apoptotic stimuli than subcutaneous preadendipocytes, demonstrating another intrinsic site-specific difference between human adipose cells of the two depots, and suggesting that the regulation of adipose tissue distribution in humans could involve depot-specific differences in rates ofPreadipocyte apoptosis.

Catecholamine stimulated lipolysis in differentiated human preadipocytes in a serum‐free, defined medium

The authors' medium supported the differentiation of a very high percentage of human preadipocytes which developed a sensitive β‐adrenergic lipolytic response but which lacked an α2‐ adrenergic antilipolytic responded.

Regional variation of adipose differentiation in cultured stromal-vascular cells from the abdominal and femoral adipose tissue of obese women.

The results suggest that regional differences exist in the capacity of adipose tissue depots to form new fat cells, which may help to understand changes in adipOSE tissue distribution during adult life.

Activators of peroxisome proliferator-activated receptor gamma have depot-specific effects on human preadipocyte differentiation.

The site-specific responsiveness of human preadipocytes to TZDs may be involved in the beneficial effects of these compounds on in vivo insulin sensitivity.

Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium.

It is concluded that adult human adipose tissue still contains precursor cells that are able to undergo adipose differentiation in vitro and this improved culture system may offer the opportunity to characterize other adipogenic factors as well as antiadipogenic factors involved in the control of adipose tissues growth.

In vitro lipid synthesis in human adipose tissue from three abdominal sites.

Investigation of cellular mechanisms that might contribute to enlargement of intra-abdominal adipocytes, lipolysis, triacylglycerol (TG), and diacyl Glyceride (DG) synthesis from [14C]glucose was measured in abdominal subcutaneous, omental, and mesenteric adipose tissue from severely obese women and men.

Reconstitution of Insulin-sensitive Glucose Transport in Fibroblasts Requires Expression of Both PPARγ and C/EBPα*

The results suggest that although PPARγ is sufficient to trigger the adipogenic program, C/EBPα is required for establishment of insulin-sensitive glucose transport.
...