Faster capillary electrophoresis separation of wheat proteins through modifications to buffer composition and sample handling.

@article{Bean1998FasterCE,
  title={Faster capillary electrophoresis separation of wheat proteins through modifications to buffer composition and sample handling.},
  author={Scott R Bean and George L. Lookhart},
  journal={Electrophoresis},
  year={1998},
  volume={19 18},
  pages={3190-8}
}
Studies were conducted to produce faster, simpler, more rugged protocols for separating wheat proteins by high performance capillary electrophoresis (HPCE). Three areas were targeted for improvement: initial capillary equilibration procedures, buffer composition, and post-separation rinsing procedures. For the initial equilibration of capillaries, a brief rinse with a hydroxypropylmethylcellulose (HPMC) solution was the most critical factor for successful separation of wheat proteins. To reduce… CONTINUE READING
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