Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens

@article{Grewe2011FastTT,
  title={Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens},
  author={Benjamin F. Grewe and Fabian Friedrich Voigt and Marcel van 't Hoff and Fritjof Helmchen},
  journal={Biomedical Optics Express},
  year={2011},
  volume={2},
  pages={2035 - 2046},
  url={https://api.semanticscholar.org/CorpusID:17401027}
}
By introducing an electrically tunable lens into the excitation path of a two-photon microscope, this work was able to realize fast axial focus shifts within 15 ms and measure neuronal population activity of about 40 neurons across two imaging planes separated by 40 μm.
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