Fast purification of Phaseolus vulgaris isolectins.


Phytohemagglutinin from red kidney bean has been purified by affinity chromatography on a human alpha 1-acid glycoprotein Sepharose 4B column. Further purification of the hemagglutinin's five isolectins was achieved on a Mono S column with an 86% protein recovery. Each sequentially eluted isolectin from the ion exchange column displayed either… (More)


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@article{Zenteno1994FastPO, title={Fast purification of Phaseolus vulgaris isolectins.}, author={Efrain Zenteno and M. Ortega and Z. Qin and J P Montreuil and Henri Debray}, journal={Preparative biochemistry}, year={1994}, volume={24 3-4}, pages={175-83} }