Fast-protein liquid chromatography system as a tool for liposome preparation by the extrusion procedure.

@article{Turnek1994FastproteinLC,
  title={Fast-protein liquid chromatography system as a tool for liposome preparation by the extrusion procedure.},
  author={Jaroslav Tur{\'a}nek},
  journal={Analytical biochemistry},
  year={1994},
  volume={218 2},
  pages={352-7}
}
Employment of fast-protein liquid chromatography (FPLC) system and a high-pressure filtration cell is described for the production of liposomes by the rapid extrusion technique. The system allows processing of up to 50 ml liposomal suspension in one batch at a defined temperature and applied pressure of up to 4.5 MPa. This relatively simple technique is applicable in any laboratory equipped with FPLC or HPLC systems.