Corpus ID: 34022840

Fast protein liquid chromatography (FPLC) of leukaemic cell N-acetyl beta-D hexosaminidases.

@article{Scott1987FastPL,
  title={Fast protein liquid chromatography (FPLC) of leukaemic cell N-acetyl beta-D hexosaminidases.},
  author={Colin Stephen Scott and M. Patel and Allistair N. Stark and B. E. Roberts},
  journal={Leukemia research},
  year={1987},
  volume={11 5},
  pages={
          437-44
        }
}
Leukaemic myeloid and lymphoid cell N-acetyl beta-D glucosaminidase (hexosaminidase) enzymes were fractionated by Fast Protein Liquid Chromatography (FPLC) using high-resolution ion exchange (Mono-S and Mono-Q), gel filtration (Superose-6) and chromatofocusing (Mono-P) columns. Although only one molecular weight species was detected in haemopoietic cells, with an apparent mass of 129 kD, "isoenzyme" variants defined by differences in molecular charge were considerably more diverse than… Expand