Fast protein-depletion system utilizing tetracycline repressible promoter and N-end rule in yeast

@inproceedings{Gnanasundram2015FastPS,
  title={Fast protein-depletion system utilizing tetracycline repressible promoter and N-end rule in yeast},
  author={Sivakumar Vadivel Gnanasundram and Martin Kos},
  booktitle={Molecular biology of the cell},
  year={2015}
}
A protein depletion by promoter shutoff or protein destabilization is an important tool in investigation of functions of essential genes. Various approaches using different repressible promoters, inducible degrons, or their combinations were developed. While successful, the current techniques have a drawback in that they require fusion of a large degradation tag to the target protein and/or a change in growth conditions to repress the promoter. We describe efficient protein depletion using the… CONTINUE READING
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