Fast multiplexed polymerase chain reaction for conventional and microfluidic short tandem repeat analysis.

@article{Giese2009FastMP,
  title={Fast multiplexed polymerase chain reaction for conventional and microfluidic short tandem repeat analysis.},
  author={Heidi Giese and Roger Lam and Richard F. Selden and Eugene K. B. Tan},
  journal={Journal of forensic sciences},
  year={2009},
  volume={54 6},
  pages={1287-96}
}
The time required for short tandem repeat (STR) amplification is determined by the temperature ramp rates of the thermal cycler, the components of the reaction mix, and the properties of the reaction vessel. Multiplex amplifications in microfluidic biochip-based and conventional tube-based thermal cyclers have been demonstrated in 17.3 and 19 min… CONTINUE READING