• Corpus ID: 82028037

Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII

@inproceedings{Shi2010FastMG,
  title={Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII},
  author={Zhenyu Shi and Teng Li and Guo-Qiang Chen},
  year={2010}
}
25 October 2010 1. Purpose With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on Type II restriction enzyme DraIII. 2. Relation to other BBF RFCs This BBF RFC updates BBF RFC 10. 3. Copyright Notice Copyright (C) The BioBricks Foundation (2010). All… 
1 Citations
BBF RFC 105: The Intein standard - a universal way to modify proteins after translation
1 Purpose This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology

References

SHOWING 1-3 OF 3 REFERENCES
Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Author's Contact Information Zhenyu SHI errisy@gmail
  • Author's Contact Information Zhenyu SHI errisy@gmail