Fast manipulation of cellular cAMP level by light in vivo

@article{SchrderLang2007FastMO,
  title={Fast manipulation of cellular cAMP level by light in vivo},
  author={Saskia Schr{\"o}der-Lang and M. Schw{\"a}rzel and Reinhard Seifert and T. Str{\"u}nker and S. Kateriya and Jens Looser and Masakatsu Watanabe and U. Kaupp and P. Hegemann and G. Nagel},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={39-42}
}
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACα and PACβ. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells. 
Photoactivated Adenylyl Cyclases: Fundamental Properties and Applications.
TLDR
PAC is a flavoprotein that catalyzes the production of cAMP upon illumination with blue light, which enables us to optogenetically manipulate intracellular cAMP levels in various biological systems. Expand
Photoactivatable adenylyl cyclases (PACs) as a tool to study cAMP signaling in vivo: an overview.
TLDR
The functional features of different cyclases are considered and operating guidelines to optimize the use of PACs in vivo are provided and two different PAC transgenes-the euglenoid euPACα and eu PACβ, as well as the b eggiatoan bPac-are available. Expand
Mechanistic insights in light-induced cAMP production by photoactivated adenylyl cyclase alpha (PACα)
TLDR
It is reported that the action spectrum of PACα is flavoprotein-typical, with maxima at ∼380 and ∼470 nm and an intermolecular dimerization of C1 and C2 domains on PACα for a functional enzyme. Expand
Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium
Significance Optogenetics is a rapidly growing field in which light is used to control biological systems. We show that Oscillatoria acuminata photoactivated adenylate cyclase (OaPAC) proteinExpand
Characterization and engineering of photoactivated adenylyl cyclases
TLDR
This work engineered derivatives of the red-light-sensitive PAC with altered responses to light, with one variant, denoted DdPAC, showing enhanced response to light and stand to enrich the optogenetic toolkit and thus facilitate the detailed analysis of cNMP metabolism and signaling. Expand
BIOCHEMICAL CHARACTERIZATION OF PHOTOACTIVATED ADENYLYL CYCLASE FROM NAEGLERIA GRUBERI
Blue light sensors using FAD (BLUF) domains are flavin based blue light photoreceptors. The BLUF domains are often fused with various effector domains. BLUF domain coupled with adenylyl cyclaseExpand
Korrigierte Fassung A photoactivated adenylyl cyclase as an optogenetic tool to manipulate neuronal signaling and synaptic plasticity Dissertation
The recent success of Channelrhodopsin (ChR) in optogenetics has caused increasing interest also in enzymes that are directly activated by light. We have identified in the genome of the bacteriumExpand
Light Modulation of Cellular cAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, bPAC, of the Soil Bacterium Beggiatoa*♦
The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium BeggiatoaExpand
A unique choanoflagellate enzyme rhodopsin exhibits light-dependent cyclic nucleotide phosphodiesterase activity
TLDR
An unusual microbial rhodopsin found in the choanoflagellate Salpingoeca rosetta and contains a C-terminal cyclic nucleotide phosphodiesterase (PDE) domain that may hold promise as a potential optogenetic tool for light control of intracellular cyclicucleotides. Expand
How to control cyclic nucleotide signaling by light.
TLDR
The optogenetic toolkit now allows to control cyclic nucleotide-dependent signaling by light in vitro and in vivo and a multitude of other cellular functions. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 29 REFERENCES
A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis
TLDR
A new type of blue-light receptor flavoprotein, photoactivated adenylyl cyclase, is found in the photoreceptor organelle of Euglena gracilis, with molecular genetic evidence that it mediates the step-up photophobic response. Expand
Kinetic analysis of the activation of photoactivated adenylyl cyclase (PAC), a blue-light receptor for photomovements of Euglena.
TLDR
It is concluded that an increase in intracellular cAMP levels evoked by photoactivation of PAC is a key event of the step-up photophobic response. Expand
Regulation and organization of adenylyl cyclases and cAMP.
  • D. Cooper
  • Biology, Medicine
  • The Biochemical journal
  • 2003
TLDR
Microdomains are a consequence of these solutions, in which cAMP dynamics may differ from the broad cytosol, and are beginning to reveal cAMP fluctuations in these various compartments. Expand
Light Activation of Channelrhodopsin-2 in Excitable Cells of Caenorhabditis elegans Triggers Rapid Behavioral Responses
TLDR
Channelrhodopsin-2 (ChR2) is employed, a directly light-gated cation channel from the green alga Chlamydomonas reinhardtii, in excitable cells of the nematode Caenorhabditis elegans, to trigger specific behaviors, simply by illumination, in neurons and muscles. Expand
Channelrhodopsin-2 and optical control of excitable cells
TLDR
Technological issues relevant to the temporal precision, spatial targeting and physiological implementation of ChR2, in the context of other photostimulation approaches to optical control of excitable cells are explored. Expand
Selective Histamine Uptake Rescues Photo- and Mechanoreceptor Function of Histidine Decarboxylase-Deficient DrosophilaMutant
TLDR
It is speculated that histamine of intrinsic neurons may function as a neuromodulator rather than as a fast transmitter, because a set of CNS-intrinsic neurons that in the wild type contain high concentrations of histamine apparently lacks this uptake mechanism. Expand
A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell
TLDR
It is found that prostaglandin E1 stimulation of human embryonic kidney cells caused a transient increase in cAMP concentration near the membrane, and in the same time frame, the total cellular cAMP rose to a steady level. Expand
Control of ligand specificity in cyclic nucleotide-gated channels from rod photoreceptors and olfactory epithelium.
TLDR
It is concluded that the hydroxyl group of Thr-560 (rod) and Thr-537 (olfactory) forms an additional hydrogen bond with cGMP, but not cAMP, and thereby provides the structural basis for ligand discrimination in cyclic nucleotide-gated channels. Expand
Hydrogen-bond switching through a radical pair mechanism in a flavin-binding photoreceptor.
TLDR
This work shows that photoactivation of BLUF domains proceeds by means of a radical-pair mechanism, driven by electron and proton transfer from the protein to the flavin, resulting in the transient formation of anionic and neutral flavin radical species that finally result in the long-lived signaling state on a 100-ps timescale. Expand
Structure of a novel photoreceptor, the BLUF domain of AppA from Rhodobacter sphaeroides.
TLDR
The flavin-binding BLUF domain of AppA represents a new class of blue light photoreceptors that are present in a number of bacterial and algal species and demonstrates a new function for the common ferredoxin-like fold. Expand
...
1
2
3
...