Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

@article{Ladoux2000FastKO,
  title={Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.},
  author={Beno{\^i}t Ladoux and Jean-Pierre Quivy and Patrick S Doyle and Olivia du Roure and Genevi{\`e}ve Almouzni and J. L. Viovy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 26},
  pages={14251-6}
}
Fluorescence videomicroscopy and scanning force microscopy were used to follow, in real time, chromatin assembly on individual DNA molecules immersed in cell-free systems competent for physiological chromatin assembly. Within a few seconds, molecules are already compacted into a form exhibiting strong similarities to native chromatin fibers. In these extracts, the compaction rate is more than 100 times faster than expected from standard biochemical assays. Our data provide definite information… CONTINUE READING