Fast kinetics of calcium release induced by myo-inositol trisphosphate in permeabilized rat hepatocytes.

@article{Champeil1989FastKO,
  title={Fast kinetics of calcium release induced by myo-inositol trisphosphate in permeabilized rat hepatocytes.},
  author={Philippe Champeil and Laurent Combettes and Brigitte Sandrin Berthon and Edith Doucet and St{\'e}phane Orlowski and Michel Claret},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 30},
  pages={17665-73}
}
We used a stopped-flow method for determining the kinetic properties (between 10 ms and 10 s) of the Ca2+ release induced by inositol 1,4,5-trisphosphate (InsP3) in saponin-treated rat hepatocytes. Preliminary experiments ensured that the indicator was able to monitor rapid changes in free Ca2+ reliably. At 20 degrees C, a maximally efficient concentration of 10 microM InsP3 released Ca2+ with a half-time of 150-300 ms, the initial rate being about 1-2 nmol of Ca2+/mg of cell protein/s. The… CONTINUE READING