Fast isogenic mapping-by-sequencing of ethyl methanesulfonate-induced mutant bulks.

@article{Hartwig2012FastIM,
  title={Fast isogenic mapping-by-sequencing of ethyl methanesulfonate-induced mutant bulks.},
  author={Benjamin J. Hartwig and Geo Velikkakam James and Kathryn Konrad and Korbinian Schneeberger and Franziska Turck},
  journal={Plant physiology},
  year={2012},
  volume={160 2},
  pages={591-600}
}
Mapping-by-sequencing (or SHOREmapping) has revitalized the powerful concept of forward genetic screens in plants. However, as in conventional genetic mapping approaches, mapping-by-sequencing requires phenotyping of mapping populations established from crosses between two diverged accessions. In addition to the segregation of the focal phenotype, this introduces natural phenotypic variation, which can interfere with the recognition of quantitative phenotypes. Here, we demonstrate how mapping… CONTINUE READING
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