Fast filtration for metabolome sampling of suspended animal cells

@article{Volmer2010FastFF,
  title={Fast filtration for metabolome sampling of suspended animal cells},
  author={Martin Volmer and Stefan Northoff and Sebastian Scholz and Tobias Th{\"u}te and Heino B{\"u}ntemeyer and Thomas Noll},
  journal={Biotechnology Letters},
  year={2010},
  volume={33},
  pages={495-502}
}
A new method for sampling suspended animal cells by fast filtration is presented that allows rapid quenching of cellular metabolism and efficient separation of the cells from culture medium. Compared to sampling with a microstructure heat exchanger or centrifugation without prior quenching, the adenylate energy charge and the measured concentrations especially of metabolites with a high turnover rate or of metabolites early in metabolic pathways were substantially higher. No leakage of ATP from… CONTINUE READING