Fast atom bombardment mass spectrometric quantitative analysis of methionine-enkephalin in human pituitary tissues.

@article{Kusmierz1990FastAB,
  title={Fast atom bombardment mass spectrometric quantitative analysis of methionine-enkephalin in human pituitary tissues.},
  author={J J Kusmierz and R A Sumrada and Dominic M. Desiderio},
  journal={Analytical chemistry},
  year={1990},
  volume={62 21},
  pages={2395-400}
}
Picomole amounts of endogenous methionine-enkephalin (ME = YGGFM) were quantified in 11 individual human pituitaries by fast atom bombardment mass spectrometry methods. Quantification was based either upon the comparison of the molecular ion (MH+) current of endogenous ME versus the current of a deuterated ME internal standard (d5-ME) or, similarly, upon the unimolecular decomposition MH+----YGGF-+ In the first field-free region to produce the unique tetrapeptide fragment ion. The latter method… CONTINUE READING
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