Fast SARS-CoV-2 detection protocol based on RNA precipitation and RT-qPCR in nasopharyngeal swab samples

@article{Guruceaga2020FastSD,
  title={Fast SARS-CoV-2 detection protocol based on RNA precipitation and RT-qPCR in nasopharyngeal swab samples},
  author={Xabier Guruceaga and Amanda Sierra and Daniel Marino and Izortze Santin and Jon Ander Nieto-Garai and Jose Ram{\'o}n Bilbao and Maier Lorizate and Patricia Aspichueta and Adhara Gaminde-Blasco and Adri{\'a}n Bozal-Leorri and Agust{\'i}n J. Mar{\'i}n-Pe{\~n}a and Ainara Castellanos-Rubio and Ainhoa Iglesias-Ara and Aitor Rementeria and Ana Bernal‐Chico and Andoni Ramirez-Garcia and Ane Olazagoitia-Garmendia and Asier Benito-Vicente and C{\'e}sar Mart{\'i}n and Eider Bilbao Castellanos and Eneritz Rueda-Ala{\~n}a and Fabio Cavaliere and Guiomar P{\'e}rez de Nanclares and Igor Aurrekoetxea and Iraia Garc{\'i}a-Santisteban and Irantzu Bernales and Itziar Gonzalez-Moro and Jan T{\o}nnesen and Jimena Baleriola and Jon Vallejo-Rodr{\'i}guez and Leire Aparicio-Fernandez and Leire Martin-Souto and Luis Manuel Mendoza and Maialen Areitio and Maialen Sebastian-delaCruz and Maren Ortiz-Zarragoitia and Mari Paz Serrano-Regal and Miren Basaras and Nora Fernandez-Jimenez and Olatz Pampliega and S. Alegre and Susi Marcos and Teresa Fuertes-Mendiz{\'a}bal and Unai Galicia-Garc{\'i}a and Uxue Perez-Cuesta and Ver{\'o}nica Torrano and Virginia Sierra-Torre and Xabier Buqu{\'e} and Ugo Mayor},
  journal={medRxiv},
  year={2020}
}
The SARS-CoV-2 pandemic has evolved far more aggressively in countries lacking a robust testing strategy to identify infected individuals. Given the global demand for fast and reliable diagnosis to determine the carrier individuals, a stock-out scenario for a number of essential reagents/kits used along the diagnostic process has been foreseen by many organizations. Having identified the RNA extraction step as one of the key bottlenecks, we tested several alternatives that avoid the use of… Expand
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A magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing and can be quickly set up in a laboratory without access to an automated pipetting robot is provided. Expand
SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP
  • Steffen Klein, Thorsten G. Müller, +23 authors P. Chlanda
  • Chemistry, Medicine
  • medRxiv
  • 2020
TLDR
A magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing and can be quickly set up in a laboratory without access to an automated pipetting robot is provided. Expand
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TLDR
PEARL is a fast, low-cost, and simple method that uses common laboratory reagents and offers comparable performance to commercial RNA extraction kits to streamline the detection of DNA and RNA viruses, including SARS-CoV-2. Expand
A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2
TLDR
PEARL offers an alternative method to isolate host and pathogen nucleic acids and proteins to streamline the detection of DNA and RNA viruses, including SARS-CoV-2. Expand
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