Fast, triangular voltage clamp for recording and kinetic analysis of an ion transporter expressed in Xenopus oocytes.

Abstract

We present a procedure for determination of 11 system parameters of an ion transporter expressed in Xenopus oocytes. The experiments consist of fast triangular voltage-clamp experiments in the presence and absence of external substrate. A four-state enzymatic cycle operating between an external and an internal section of electrodiffusion is used for… (More)

Topics

9 Figures and Tables

Cite this paper

@article{Gradmann2005FastTV, title={Fast, triangular voltage clamp for recording and kinetic analysis of an ion transporter expressed in Xenopus oocytes.}, author={Dietrich Gradmann and Carl M. Boyd}, journal={Biophysical journal}, year={2005}, volume={89 1}, pages={734-44} }