Fast, high precision autofocus on a motorised microscope: Automating blood sample imaging on the OpenFlexure Microscope.

  title={Fast, high precision autofocus on a motorised microscope: Automating blood sample imaging on the OpenFlexure Microscope.},
  author={Joe Knapper and Joel T. Collins and Julian Stirling and Samuel Mcdermott and William G Wadsworth and Richard W. Bowman},
  journal={Journal of microscopy},
The OpenFlexure Microscope is a 3D printed, low-cost microscope capable of automated image acquisition through the use of a motorised translation stage and a Raspberry Pi imaging system. This automation has applications in research and healthcare, including in supporting the diagnosis of malaria in low resource settings. The plasmodium parasites which cause malaria require high magnification imaging, which has a shallow depth of field, necessitating the development of an accurate and precise… Expand
1 Citations

Figures from this paper

Simplifying the OpenFlexure microscope software with the web of things
Using a Web of Things approach has resulted in a user-friendly and extremely versatile software control solution for the OpenFlexure Microscope, and it is believed this approach could be generalized in the future to make automated experiments involving several instruments much easier to implement. Expand


Robotic microscopy for everyone: the OpenFlexure Microscope
The OpenFlexure Microscope has been designed to enable low-volume manufacturing and maintenance by local personnel, vastly increasing accessibility and demonstrating that local manufacturing can be a viable alternative to international supply chains that can often be costly, slow, and unreliable. Expand
Autofocusing in computer microscopy: Selecting the optimal focus algorithm
This article presents a comprehensive comparison study of 18 focus algorithms in which a total of 139,000 microscope images were analyzed and guidelines allow for the selection of the optimal focus algorithm for different microscopy applications. Expand
Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork
“Microscopi” is developed, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Expand
Globally optimal stitching of tiled 3D microscopic image acquisitions
This work developed a method that, based on the Fourier Shift Theorem, computes all possible translations between pairs of 3D images, yielding the best overlap in terms of the cross-correlation measure and subsequently finds the globally optimal configuration of the whole group of3D images. Expand
Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation
TIE imaging is suggested as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index/OPL, and potential applications in biogeochemical modelling and climate sciences are suggested. Expand
Foldscope: Origami-Based Paper Microscope
An ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes that can survive harsh field conditions while providing a diversity of imaging capabilities. Expand
Auto-aligning stimulated emission depletion microscope using adaptive optics.
Adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm, enabling diffraction-unlimited resolution in fluorescence microscopy at the nanoscale. Expand
Multifocus color image sequence fusion based on mean shift segmentation.
The proposed region-based technique for fusion of a multifocus color image sequence in the LUV color space can be used to extend the depth of field (DOF) of a camera system effectively. Expand
UC2 – A 3D-printed General-Purpose Optical Toolbox for Microscopic Imaging
A novel open-source modular electro-optical toolbox to be used for many different optical setups is presented and a live-cell imaging device and an optical super-resolution microscope are shown using Image-Scanning-Microscopy techniques. Expand
The JPEG still picture compression standard
The Baseline method has been by far the most widely implemented JPEG method to date, and is sufficient in its own right for a large number of applications. Expand