Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).

  title={Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).},
  author={Thomas Dertinger and Robert Colyer and Gopalakrishnan Iyer and Shimon Weiss and J{\"o}rg Enderlein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={106 52},
Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of overcoming the fundamental diffraction limit of light. We present here an approach for obtaining subdiffraction limit optical resolution in all three dimensions. This method relies on higher-order statistical analysis of temporal fluctuations (caused by… CONTINUE READING
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