Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy.

@article{Morozova2010FarredFP,
  title={Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy.},
  author={Kateryna S. Morozova and Kiryl D. Piatkevich and Travis J. Gould and Jinghang Zhang and Joerg Bewersdorf and Vladislav V Verkhusha},
  journal={Biophysical journal},
  year={2010},
  volume={99 2},
  pages={L13-5}
}
Far-red fluorescent proteins are required for deep-tissue and whole-animal imaging and multicolor labeling in the red wavelength range, as well as probes excitable with standard red lasers in flow cytometry and fluorescence microscopy. Rapidly evolving superresolution microscopy based on the stimulated emission depletion approach also demands genetically encoded monomeric probes to tag intracellular proteins at the molecular level. Based on the monomeric mKate variant, we have developed a far… CONTINUE READING