False positives and false negatives measure less than 0.001% in labeling ssDNA with osmium tetroxide 2,2’-bipyridine

@article{Kanavarioti2016FalsePA,
  title={False positives and false negatives measure less than 0.001\% in labeling ssDNA with osmium tetroxide 2,2’-bipyridine},
  author={Anastassia Kanavarioti},
  journal={Beilstein Journal of Nanotechnology},
  year={2016},
  volume={7},
  pages={1434 - 1446}
}
  • A. Kanavarioti
  • Published 12 October 2016
  • Biology
  • Beilstein Journal of Nanotechnology
Osmium tetroxide 2,2’-bipyridine (OsBp) is known to react with pyrimidines in ssDNA and preferentially label deoxythymine (T) over deoxycytosine (C). The product, osmylated DNA, was proposed as a surrogate for nanopore-based DNA sequencing due to OsBp’s “perfect” label attributes. Osmylated deoxyoligos translocate unassisted and measurably slow via sub-2 nm SiN solid-state nanopores, as well as via the alpha-hemolysin (α-HL) pore. Both nanopores discriminate clearly between osmylated and intact… 
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