Failure to detect the extracellular calcium-sensing receptor (CasR) in human osteoblast cell lines.

@article{Pi1999FailureTD,
  title={Failure to detect the extracellular calcium-sensing receptor (CasR) in human osteoblast cell lines.},
  author={Min Pi and Todd K. Hinson and Leigh Quarles},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={1999},
  volume={14 8},
  pages={
          1310-9
        }
}
Whether the known calcium-sensing receptor (CasR) is present in osteoblasts is a source of considerable controversy. Prior studies failed to detect CasR in osteoblasts, but more recent investigations purport the detection of CasR in several osteoblast cell lines by immunoblot analysis with polyclonal anti-CasR antisera (4637) and low stringency reverse transcriptase-polymerase chain reaction (RT-PCR). To explain these disparate findings, we performed immunoblot analysis with the 4637 anti-CasR… CONTINUE READING
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