Factors responsible for target site selection in Tn10 transposition: a role for the DDE motif in target DNA capture.

  title={Factors responsible for target site selection in Tn10 transposition: a role for the DDE motif in target DNA capture.},
  author={Murray S. Junop and David B. Haniford},
  journal={The EMBO journal},
  volume={16 10},
Tn10, like several other transposons, exhibits a marked preference for integration into particular target sequences. Such sequences are referred to as integration hotspots and have been used to define a consensus target site in Tn10 transposition. We demonstrate that a Tn10 hotspot called HisG1, which was identified originally in vivo, also functions as an integration hotspot in vitro in a reaction where the HisG1 sequence is present on a short DNA oligomer. We use this in vitro system to… CONTINUE READING


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