Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA-RNA recombination.

@article{Kim2001FactorsRT,
  title={Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA-RNA recombination.},
  author={Min Ja Kim and Cheng Kao},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2001},
  volume={98 9},
  pages={4972-7}
}
Copy-choice RNA recombination occurs during viral RNA synthesis when the viral transcription complex switches templates. We demonstrate that RNA-dependent RNA polymerase from bovine viral diarrhea virus and the replicases from three plant-infecting RNA viruses can produce easily detectable recombination products in vitro by switching templates during elongative RNA synthesis. Template sequence and/or structure, and NTP availability affected the frequency of template switch by the transcription… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 48 extracted citations

Diverse Mechanisms of RNA Recombination

Molecular Biology • 2005
View 7 Excerpts
Highly Influenced

Mechanisms and consequences of positive-strand RNA virus recombination.

The Journal of general virology • 2018
View 7 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…