Factors influencing transformation frequency of tomato (Lycopersicon esculentum)

@article{Roekel2004FactorsIT,
  title={Factors influencing transformation frequency of tomato (Lycopersicon esculentum)},
  author={J. S. C. Roekel and B. Damm and L. Melchers and A. Hoekema},
  journal={Plant Cell Reports},
  year={2004},
  volume={12},
  pages={644-647}
}
  • J. S. C. Roekel, B. Damm, +1 author A. Hoekema
  • Published 2004
  • Biology, Medicine
  • Plant Cell Reports
  • We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that Agrobacterium strains harbouring a L,L-succinamopine type helper plasmid yielded significantly higher transformation frequencies than those with octopine or nopaline type helper… CONTINUE READING
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