Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo

@article{Niedz2004FactorsIS,
  title={Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo},
  author={Randall P. Niedz and Sandra Schiller Smith and Kerry B. Dunbar and Christine T. Stephens and Harry H. Murakishi},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={18},
  pages={313-319}
}
Cotyledonary explants of 4-day-oldCucumis melo cv. ‘Hale's Best Jumbo’ in vitro seedlings showed maximum initiation of shoot buds when cultured onto a revised Murashige & Skoog medium supplemented with 5 μM indole-3-acetic acid and 5 μM benzylaminopurine and cultured at 25–29°C under low light intensity (5–30 μmol m-2 s-1). Subculture of the shoot buds onto the same medium without auxin and supplemented with 3 μM benzylaminopurine caused the development of shoots from 30% of the buds. The… CONTINUE READING