In vitro regeneration of clonally uniform plants of Crataeva magna: a high value medicinal tree by axillary branching method
Success at propagating peach (Prunus persica (L.) Batsch) scion cultivars in vitro has been limited. This study describes factors influencing in vitro multiplication and rooting of 8 peach scion cultivars and one rootstock, as well as acclimatization and genetic stability of these cultivars. Shoot multiplication was best when 8.8 μM 6-benzylamino purine (BA) was added to the shoot proliferation medium. Maximum rooting occurred when shoots were placed on 1/2-strength Murashige and Skoog (MS) medium, stored in the dark at 4°C for 35 to 40 days and then incubated on rooting medium in the dark at 26°C for 14 days. All cultivars exposed to 1/2-strength MS medium supplemented with 28.5μM of either indoleacetic acid (IAA), indolebutyric acid (IBA) or α-napthaleneacetic acid (NAA) rooted best on NAA medium. A 5-fold reduction in NAA concentration to 5.8 μM, the use of IAA plus phenolic rooting cofactors, and length of time shoots were in vitro prior to rooting each increased the percentage of rooting for most cultivars. No plant loss occurred during acclimatization. Cytogenetic analysis of micropropagated plants indicated that all plants were diploid, 2n=2x=16. Examination of the performance of in vitro propagated plants under field conditions is now in progress.