Factors influencing heat-labile Escherichia coli enterotoxin activity.

@article{Mundell1976FactorsIH,
  title={Factors influencing heat-labile Escherichia coli enterotoxin activity.},
  author={D H Mundell and C R Anselmo and R M Wishnow},
  journal={Infection and immunity},
  year={1976},
  volume={14 2},
  pages={
          383-8
        }
}
In this study, conditions for production, detection, and storage of heat-labile Escherichia coli enterotoxin (LT) in culture filtrates from E. coli H-10407 were defined by using the adrenal tumor cell assay system. An enriched medium containing 0.6% yeast extract, 2% Casamino Acids, and 0.25% glucose buffered at pH 8.5 produced the highest LT activity of the various test media. In E. coli strain H-10407, LT activity was markedly decreased if the initial pH of the culture media was reduced to pH… CONTINUE READING
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