Factors influencing antibody stability at solid-liquid interfaces in a high shear environment.

@article{Biddlecombe2009FactorsIA,
  title={Factors influencing antibody stability at solid-liquid interfaces in a high shear environment.},
  author={James G Biddlecombe and Gemma Smith and Shahid Uddin and Sandrine Mulot and David Anthony Spencer and Chris Van Gee and Brendan Cormick Fish and Daniel G Bracewell},
  journal={Biotechnology progress},
  year={2009},
  volume={25 5},
  pages={
          1499-507
        }
}
A rotating disk shear device was used to study the effect of interfacial shear on the structural integrity of human monoclonal antibodies of IgG4 isotype. Factors associated with the solution conditions (pH, ionic strength, surfactant concentration, temperature) and the interface (surface roughness) were studied for their effect on the rate of IgG4 monomer loss under high shear conditions. The structural integrity of the IgG4 was probed after exposure to interfacial shear effects by SDS-PAGE… CONTINUE READING
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