Factors influencing adenovirus-mediated airway transduction in fetal mice.

  title={Factors influencing adenovirus-mediated airway transduction in fetal mice.},
  author={Suzanne M. K. Buckley and Simon N Waddington and Sarah Jezzard and Lorraine Lawrence and Holm Schneider and Maxine V. Holder and Michael Themis and Charles Coutelle},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  volume={12 3},
Intra-amniotic injection of adenovirus allows transduction of the fetal airways following natural fetal breathing movements. This administration method is promising for use in gene therapy for cystic fibrosis and other diseases for which the main target for exogenous gene expression is the lung. Here we have investigated factors that may affect the efficacy of gene transfer to the murine fetal lung. We examined marker compound distribution and transgene expression (from a first-generation… CONTINUE READING