Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.

@article{Gursinsky2008FactorsAS,
  title={Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.},
  author={Torsten Gursinsky and Daniel Gr{\"o}be and Angelika Schierhorn and Jana J{\"a}ger and Jan Remmer Andreesen and Brigitte S{\"o}hling},
  journal={Applied and environmental microbiology},
  year={2008},
  volume={74 5},
  pages={
          1385-93
        }
}
Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of selenocysteine into the nascent polypeptide chain. The synthesis of three different selenoproteins from the gram-positive anaerobe Eubacterium acidaminophilum in E. coli was studied… CONTINUE READING

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