Factors affecting the survival of frozen-thawed mouse spermatozoa.

@article{An2000FactorsAT,
  title={Factors affecting the survival of frozen-thawed mouse spermatozoa.},
  author={Taichen An and Masahiko Iwakiri and Keisuke Edashige and Toshihiro Sakurai and Magosaburo Kasai},
  journal={Cryobiology},
  year={2000},
  volume={40 3},
  pages={
          237-49
        }
}
Mouse epididymal spermatozoa were frozen in solutions containing various compounds with different molecular weights, and the factors affecting the postthawing survival were examined. Monosaccharides (glucose, galactose) had almost no protective effect regardless of the concentration and the temperature of exposure. On the other hand, disaccharides (sucrose, trehalose) and trisaccharides (raffinose, melezitose) resulted in higher survival rates, especially at a concentration of around 0.35 mol… CONTINUE READING
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