Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli

  title={Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli},
  author={Anne Usvalampi and Kristiina J Kiviharju and Matti S. A. Leisola and Antti Nyyss{\"o}l{\"a}},
  journal={Journal of Industrial Microbiology & Biotechnology},
Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental… CONTINUE READING

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