Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.

@article{Brinster1985FactorsAT,
  title={Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.},
  author={Ralph L. Brinster and Howard Y. Chen and Myrna E. Trumbauer and M K Yagle and Richard D Palmiter},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1985},
  volume={82 13},
  pages={4438-42}
}
Microinjection of foreign DNA into fertilized mammalian eggs is a convenient means of introducing genes into the germ line. Some of the more important parameters that influence successful integration of foreign DNA into mouse chromosomes are described. The effects of DNA concentration, size, and form (supercoiled vs. linear with a variety of different ends) are considered as well as the site of injection (male pronucleus, female pronucleus, or cytoplasm) and buffer composition. The optimal… CONTINUE READING
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