Factors affecting the developmental competence of mouse oocytes grown in vitro: follicle-stimulating hormone and insulin.

@article{Eppig1998FactorsAT,
  title={Factors affecting the developmental competence of mouse oocytes grown in vitro: follicle-stimulating hormone and insulin.},
  author={John J. Eppig and Marilyn O'Brien and Frank L Pendola and Satoko Watanabe},
  journal={Biology of reproduction},
  year={1998},
  volume={59 6},
  pages={1445-53}
}
This study was undertaken to test the hypothesis that FSH treatment of cultured oocyte-granulosa cell complexes promotes acquisition of competence to complete preimplantation embryo development. Oocyte-granulosa cell complexes were isolated from the preantral follicles of 12-day-old mice and cultured for 10 days in serum-free medium, supplemented with insulin (5 microgram/ml), transferrin (5 microgram/ml), and selenium (5 ng/ml) and containing a highly potent preparation of FSH (0-5 ng/ml… CONTINUE READING

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