Factors affecting the cryosurvival of mouse two-cell embryos.


A series of 4 experiments was conducted to examine factors affecting the survival of frozen-thawed 2-cell mouse embryos. Rapid addition of 1.5 M-DMSO (20 min equilibration at 25 degrees C) and immediate, rapid removal using 0.5 M-sucrose did not alter the frequency (mean +/- s.e.m.) of blastocyst development in vitro when compared to untreated controls (90… (More)


Cite this paper

@article{Critser1988FactorsAT, title={Factors affecting the cryosurvival of mouse two-cell embryos.}, author={John K. Critser and Broderic Arneson and D V Aaker and A R Huse-Benda and G D Ball}, journal={Journal of reproduction and fertility}, year={1988}, volume={82 1}, pages={27-33} }