Factors affecting oligomerization status of UDP-glucose pyrophosphorylase.

  title={Factors affecting oligomerization status of UDP-glucose pyrophosphorylase.},
  author={Leszek A. Kleczkowski and Françoise Martz and Malgorzata Wilczynska},
  volume={66 24},
UDP-glucose pyrophosphorylase (UGPase) is involved in the production of UDP-glucose, a key precursor to polysaccharide synthesis in all organisms. UGPase activity has recently been proposed to be regulated by oligomerization, with monomer as the active species. In the present study, we investigated factors affecting oligomerization status of the enzyme, using purified recombinant barley UGPase. Incubation of wild-type (wt) UGPase with phosphate or Tris buffers promoted oligomerization, whereas… CONTINUE READING


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