Factors affecting high-frequency embryo formation inab initio pollen cultures ofNicotiana

Abstract

Consistent high-frequency embryo formation, up to 30% of the cultured pollen, was possible when embryogenic pollen were selectively isolated from the gametophytic pollen on centrifugation, at 12–15 °C, in Percoll diluted by a low level of sucrose (4%). Higher level of sucrose in separation medium was inhibitory for embryogenesis. The embryogenic pollen were… (More)
DOI: 10.1007/BF01279833

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