Factors affecting filament velocity in in vitro motility assays and their relation to unloaded shortening velocity in muscle fibers.

@article{Homsher1993FactorsAF,
  title={Factors affecting filament velocity in in vitro motility assays and their relation to unloaded shortening velocity in muscle fibers.},
  author={Earl E Homsher and Fengzhen Wang and James R Sellers},
  journal={Advances in experimental medicine and biology},
  year={1993},
  volume={332},
  pages={279-89; discussion 289-90}
}
The measurement of fluorescently labeled actin filament movement driven by mechanoenzymes (e.g. myosin) is an important methodology for the study of molecular motors. It is assumed that the filament velocity, Vf, is analogous to the unloaded shortening velocity, Vu, seen in muscle fibers. To evaluate this assumption we compared Vf to literature values for Vu with regard to the effects of [ATP], [ADP], [Pi], pH, ionic strength (10-150 mM) and temperature (15-30 degrees C). Vf and Vu are… CONTINUE READING

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