Factors affecting autocatalysis of botulinum A neurotoxin light chain.

@article{Ahmed2004FactorsAA,
  title={Factors affecting autocatalysis of botulinum A neurotoxin light chain.},
  author={S-U. Ahmed and Matthew L. Ludivico and L. A. Smith},
  journal={The protein journal},
  year={2004},
  volume={23 7},
  pages={
          445-51
        }
}
The light chain of botulinum neurotoxin serotype A undergoes autocatalytic fragmentation into two major peptides during purification and storage (Ahmed S. A. et al. 2001, J. Protein Chem. 20:221-231) by both intermolecular and intramolecular mechanisms (Ahmed S. A. et al. 2003, Biochemistry 42:12539 12549). In this study, we investigated the effects of buffers and salts on this autocatalytic reaction in the presence and absence of zinc chloride. In the presence of zinc chloride, the… CONTINUE READING
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