Micro-Analysis in Medical Biochemistry
- I.D.P. Wootton
Many workers have modified van den Bergh's method for determining bile pigment in serum (With, 1954). Two of the more satisfactory methods are those of Malloy and Evelyn (1937) and King and Coxon (1950). The present study was undertaken in order to explain differences in the results given by these two methods, and in an attempt to combine the desirable features of each, on a scale which was suitable for the day-to-day control of plasma bilirubin concentrations in newborn infants. Methods Serum.-Serum containing unconjugated, indirectreacting bilirubin was obtained from infants at exchange transfusion. Serum containing conjugated bilirubin giving the direct reaction was obtained from patients with jaundice due to stones, cirrhosis, chlorpromazine, hepatitis, and also from infants with the "inspissated bile syndrome."