Factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste.

@article{Prakash2008FactorialDF,
  title={Factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste.},
  author={Om Prakash and Mahe Talat and Syed Hadi Hasan and Rajesh Kumar Pandey},
  journal={Bioresource technology},
  year={2008},
  volume={99 16},
  pages={7565-72}
}
Free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (Cd2+) in aqueous samples. Urease from the seeds of pumpkin (Cucumis melo), being a vegetable waste, was extracted and purified to apparent homogeneity (Sp. Activity 353 U/mg protein; A280/A260=1.12) by heat treatment at 48+/-0.1 degrees C and gel filtration through Sephadex G-200. The homogeneous enzyme preparation was immobilized in 3.5% alginate leading to 86% immobilization and no leaching of… CONTINUE READING

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