Factor IX mutants with enhanced catalytic activity.

  title={Factor IX mutants with enhanced catalytic activity.},
  author={Rudolf Hartmann and Michael Dockal and W Kammlander and E Panholzer and Gerry A. F. Nicolaes and Christian Fiedler and Jan Rosing and Friedrich Scheiflinger},
  journal={Journal of thrombosis and haemostasis : JTH},
  volume={7 10},
BACKGROUND Activated coagulation factor IX (FIXa) has low catalytic activity towards its physiologic substrate FX when activated FVIII (FVIIIa) is absent. One reason for this is that the FIX surface loop 99 stabilizes FIXa in a conformation that limits access of FX to the active site. OBJECTIVES To investigate the effect of mutations in loop 99 and in the active site on FIXa activity with and without FVIIIa. METHODS Five full-length FIX mutants with amino acid exchanges in the catalytic… CONTINUE READING